Soil Geobacteraceae are the key predictors of neurotoxic methylmercury bioaccumulation in rice.

Contamination of rice by the potent neurotoxin methylmercury (MeHg) originates from microbe-mediated Hg methylation in soils. However, the high diversity of Hg methylating microorganisms in soils hinders the prediction of MeHg formation and challenges the mitigation of MeHg bioaccumulation via regulating soil microbiomes. Here we explored the roles of various cropland microbial communities in MeHg formation in the potentials leading to MeHg accumulation in rice and reveal that Geobacteraceae are the key predictors of MeHg bioaccumulation in paddy soil systems. We characterized Hg methylating microorganisms from 67 cropland ecosystems across 3,600 latitudinal kilometres. The simulations of a rice-paddy biogeochemical model show that MeHg accumulation in rice is 1.3-1.7-fold more sensitive to changes in the relative abundance of Geobacteraceae compared to Hg input, which is recognized as the primary parameter in controlling MeHg exposure. These findings open up a window to predict MeHg formation and accumulation in human food webs, enabling more efficient mitigation of risks to human health through regulations of key soil microbiomes.

Asymmetric Cell Division and Tumor Heterogeneity.

Asymmetric cell division (ACD) gives rise to two daughter cells with different fates after mitosis and is a fundamental process for generating cell diversity and for the maintenance of the stem cell population. The cancer stem cell (CSC) theory suggests that CSCs with dysregulated self-renewal and asymmetric cell division serve as a source of intra-tumoral heterogeneity. This heterogeneity complicates the diagnosis and treatment of cancer patients, because CSCs can give rise to aggressive clones that are metastatic and insensitive to multiple drugs, or to dormant tumor cells that are difficult to detect. Here, we review the regulatory mechanisms and biological significance of asymmetric division in tumor cells, with a focus on ACD-induced tumor heterogeneity in early tumorigenesis and cancer progression. We will also discuss how dissecting the relationship between ACD and cancer may help us find new approaches for combatting this heterogeneity.

Overlooked Role of Putative Non-Hg Methylators in Predicting Methylmercury Production in Paddy Soils.

Rice ingestion has been recognized as an important route of dietary exposure to neurotoxic methylmercury (MeHg) that is commonly synthesized in rice paddy soils. Although Hg methylators are known to regulate soil MeHg formation, the effect of non-Hg methylating communities on MeHg production remains unclear. Here, we collected 141 paddy soil samples from main rice-producing areas across China to identify associations between bacterial community composition (including both Hg and putative non-Hg methylators) and MeHg production. Results showed that the MeHg content in the paddy soils varied from 0.11 to 8.36 ng g-1 at a national spatial scale, which could be due to the shifts of soil microbial community composition across different areas. Our structure equation modeling suggested a strong link between bacterial community composition and MeHg content and %MeHg. More importantly, random forest analyses suggested a more significant role of putative non-Hg methylators than Hg methylators in predicting variations of soil MeHg content. The relative abundance of putative non-Hg methylators such as unclassified Xanthomonadales and Chitinophagaceae were strongly correlated with soil MeHg contents. Further, microbial network analysis revealed strong co-occurrence patterns between the putative non-Hg and Hg methylators. These findings highlight an overlooked role of non-Hg methylating communities in predicting MeHg production in paddy soils.